Effects of moderate static magnetic fields on the voltage-gated sodium and calcium channel currents in trigeminal ganglion neurons
Xiao-Wen Lu, Li Du, Liang Kou, Ning Song, Yu-Jiao Zhang, Min-Ke Wu, Jie-Fei Shen. Effects of moderate static magnetic fields on the voltage-gated sodium and calcium channel currents in trigeminal ganglion neurons. Electromagnetic Biology and Medicine..Posted online on April 8, 2014. (doi:10.3109/15368378.2014.906448).
Abstract
Aim: To study the effects of static magnetic fields (SMF) on the electrophysiological properties of voltage-gated sodium and calcium channels on trigeminal ganglion (TRG) neurons. Methods: Acutely dissociated TRG neurons of neonatal SD rats were exposed to 125-mT and 12.5-mT SMF in exposure devices and whole-cell patch-clamp recordings were carried out to observe the changes of voltage-gated sodium channels (VGSC) and calcium channels (VGCC) currents, while laser scanning confocal microscopy was used to detect intracellular free Ca2+ concentration in TRG neurons, respectively. Results: (1) No obvious change of current–voltage (I–V) relationship and the peak current densities of VGSC and VGCC currents were found when TRG neurons were exposed to 125-mT and 12.5-mT SMF. However, the activation threshold, inactivation threshold and velocity of the channel currents above were significantly altered by 125-mT and 12.5-mT SMF. (2) The fluctuation of intracellular free Ca2+ concentration within TRG neurons were slowed by 125-mT and 12.5-mT SMF. When SMF was removed, the Ca2+ concentration level showed partial recovery in the TRG neurons previously exposed by 125-mT SMF, while there was a full recovery found in 12.5-mT-SMF-exposed neurons. Conclusions: Moderate-intensity SMF could affect the electrophysiological characteristics of VGCS and VGCC by altering their activation and inactivation threshold and velocity. The fluctuations of intracellular free Ca2+ caused by SMF exposure were not permanent in TRG neurons.
http://informahealthcare.com/doi/abs/10.3109/15368378.2014.906448
The effect of pulsed electromagnetic field therapy on food sensitivity
Jean A. Monro, Basant K. Puri. The effect of pulsed electromagnetic field therapy on food sensitivity. Electromagnetic Biology and Medicine. Posted online on April 8, 2014. (doi:10.3109/15368378.2014.906450).
Abstract
Owing to the involvement of the immune system in the etiology of food sensitivity, and because pulsed electromagnetic field therapy is associated with beneficial immunologic changes, it was hypothesized that pulsed electromagnetic fields may have a beneficial effect on food sensitivity. A small pilot study was carried out in patients suffering from food sensitivity, with the antigen leukocyte antibody test being employed to index the degree of food sensitivity in terms of the number of foods to which each patient reacted. It was found that a 1-week course of pulsed electromagnetic field therapy, consisting of one hour’s treatment per day, resulted in a reduction in the mean number of reactive foods of 10.75 (p < 0.05). On the basis of these results, a larger study is warranted.
http://informahealthcare.com/doi/abs/10.3109/15368378.2014.906450
Oxidative changes and apoptosis induced by 1800-MHz electromagnetic radiation in NIH/3T3 cells
Qingxia Hou, Minglian Wang, Shuicai Wu, Xuemei Ma, Guangzhou An, Huan Liu, Fei Xie. Oxidative changes and apoptosis induced by 1800-MHz electromagnetic radiation in NIH/3T3 cells.Electromagnetic Biology and Medicine. Posted online on March 25, 2014. (doi:10.3109/15368378.2014.900507)
Abstract
To investigate the potential adverse effects of mobile phone radiation, we studied reactive oxygen species (ROS), DNA damage and apoptosis in mouse embryonic fibroblasts (NIH/3T3) after intermittent exposure (5 min on/10 min off, for various durations from 0.5 to 8 h) to an 1800-MHz GSM-talk mode electromagnetic radiation (EMR) at an average specific absorption rate of 2 W/kg. A 2′,7′-dichlorofluorescin diacetate fluorescence probe was used to detect intracellular ROS levels, immunofluorescence was used to detect γH2AX foci as a marker for DNA damage, and flow cytometry was used to measure apoptosis. Our results showed a significant increase in intracellular ROS levels after EMR exposure and it reached the highest level at an exposure time of 1 h (p < 0.05) followed by a slight decrease when the exposure continued for as long as 8 h. No significant effect on the number of γH2AX was detected after EMR exposure. The percentage of late-apoptotic cells in the EMR-exposed group was significantly higher than that in the sham-exposed groups (p < 0.05). These results indicate that an 1800-MHz EMR enhances ROS formation and promotes apoptosis in NIH/3T3 cells.
http://informahealthcare.com/doi/abs/10.3109/15368378.2014.900507
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