New Paper: EMF effects on mouse brain proteome
January 21, 2012 in -Mailing List, Cell phone news by EMFacts
Press release: January 25, 2012
The research group of Professor Lukas Margaritis (Faculty of Biology, University of Athens and the Biomedical Research Foundation of the Academy of Athens), within the framework of the activities seeking for the truth underlining the possible effects of daily life electromagnetic fields, has performed this study as part of the Doctorate Dissertation of Adamantia F. Fragopoulou.
Using ordinary working conditions of mobile phone and wireless DECT base and by applying state of the art proteome science approaches, they demonstrated that a large number of major brain proteins have been changed. Namely proteins that are responsible for the integrity of brain functions, in such critical regions like hippocampus, cerebellum and frontal lobe are below normal levels whereas an equally large number are found well above physiological levels. These “underexpressed” or “overexressed” proteins may play a role in the short term or long term effects reported as a consequence of mobile phone exposure, including memory deficits, headaches, sleep disorders, brain tumors.
This study is the first large scale analysis of the mouse brain proteome to be published so far. The research team having recently been awarded a large “Thalis” grant is potentially aiming in elucidating the EMF effects from the molecular level up to the organism level, exploiting the most suitable model systems (mice, insects, nematodes, lizards, cell cultures, human skin).
This study is the first large scale analysis of the mouse brain proteome to be published so far. The research team having recently been awarded a large “Thalis” grant is potentially aiming in elucidating the EMF effects from the molecular level up to the organism level, exploiting the most suitable model systems (mice, insects, nematodes, lizards, cell cultures, human skin).
Published in Electromagnetic Biology and Medicine, Early Online: 1–25, 2012 Copyright Q Informa Healthcare USA, Inc.
Brain proteome response following whole body exposure of mice to mobile phone or wireless DECT base radiation
Adamantia F. Fragopoulou1, Athina Samara2, Marianna H. Antonelou1, Anta Xanthopoulou3, Aggeliki Papadopoulou3, Konstantinos Vougas3, Eugenia Koutsogiannopoulou2, Ema Anastasiadou2, Dimitrios J. Stravopodis1, George Th. Tsangaris3 & Lukas H. Margaritis1
1Department of Cell Biology and Biophysics, Athens University, Athens, Greece, 2Genetics and Gene Therapy Division, Center of Basic Research II, Biomedical Research Foundation of the Academy of Athens, Athens, Greece, and 3Proteomics Research Unit, Center of Basic Research II, Biomedical Research Foundation of the Academy of Athens, Athens, Greece
Adamantia F. Fragopoulou1, Athina Samara2, Marianna H. Antonelou1, Anta Xanthopoulou3, Aggeliki Papadopoulou3, Konstantinos Vougas3, Eugenia Koutsogiannopoulou2, Ema Anastasiadou2, Dimitrios J. Stravopodis1, George Th. Tsangaris3 & Lukas H. Margaritis1
1Department of Cell Biology and Biophysics, Athens University, Athens, Greece, 2Genetics and Gene Therapy Division, Center of Basic Research II, Biomedical Research Foundation of the Academy of Athens, Athens, Greece, and 3Proteomics Research Unit, Center of Basic Research II, Biomedical Research Foundation of the Academy of Athens, Athens, Greece
Abstract:
The objective of this study was to investigate the effects of two sources of electromagnetic fields (EMFs) on the proteome of cerebellum, hippocampus, and frontal lobe in Balb/c mice following long-term whole body irradiation. Three equally divided groups of animals (6 animals/group) were used; the first group was exposed to a typical mobile phone, at a SAR level range of 0.17– 0.37 W/kg for 3 h daily for 8 months, the second group was exposed to a wireless DECT base (Digital Enhanced Cordless Telecommunications/Telephone) at a SAR level range of 0.012– 0.028 W/kg for 8 h/day also for 8 months and the third group comprised the sham-exposed animals. Comparative proteomics analysis revealed that long-term irradiation from both EMF sources altered significantly (p , 0.05) the expression of 143 proteins in total (as low as 0.003 fold downregulation up to 114 fold overexpression). Several neural function related proteins (i.e., Glial Fibrillary Acidic Protein (GFAP), Alpha-synuclein, Glia Maturation Factor beta (GMF), and apolipoprotein E (apoE)), heat shock proteins, and cytoskeletal proteins (i.e., Neurofilaments and tropomodulin) are included in this list as well as proteins of the brain metabolism (i.e., Aspartate aminotransferase, Glutamate dehydrogenase) to nearly all brain regions studied. Western blot analysis on selected proteins confirmed the proteomics data. The observed protein expression changes may be related to brain plasticity alterations, indicative of oxidative stress in the nervous system or involved in apoptosis and might potentially explain human health hazards reported so far, such as headaches, sleep disturbance, fatigue, memory deficits, and brain tumor long-term induction under similar exposure conditions.
http://www.emfacts.com/2012/01/new-paper-emf-effects-on-mouse-brain-proteome/
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